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Otto Fajardo


Date of Birth: 29th December 1979
Nationality: Peruvian

CURRENT POSITION
2009 – present        Post-doctoral fellow, FMI (Friedrich Miescher Institute for Biomedical Research), Basel (Switzerland)
                                    PI: Rainer W. Friedrich
                                    Project: Optogenetic screening of zebrafish: linking behavior to neuronal activity.           
Techniques: behavior, two-photon calcium imaging, optogenetics. Data analysis.

 

PhD

2004- 2009               PhD in Neuroscience

Institute of Neuroscience of Alicante

University Miguel Hernández/CSIC, Alicante – Spain

                                   PIs: Carlos Belmonte, Felix Viana and Roberto Gallego
                                   PhD thesis: Cold sensitivity in vagal afferents.
Techniques: calcium imaging and patch clamp in rat and mice in primary sensory neurons cultures, extracellular recordings in ex vivo nerve-organ preparations, neuronal tracing, immunohistochemistry, and behavior.

EDUCATION
2002-2004                Master in Biochemistry and Biotechnology

                                   University Francisco de Vitoria, Madrid – Spain.

Master’s Final Project: Cannabinoid receptors expression on human and rat brain.
Research Laboratory, Alcorcon Hospital, Madrid-Spain
PI: Julian Romero
Techniques: Immunohistochemistry
1997-2002                Dentistry
                                   University Federico Villarreal, Lima – Peru
Degree thesis: Antibiotic resistance in bacteria from patients with odontogenic facial infections. Hipolito Unanue Hospital, Lima – Peru.
Techniques: Bacterial cultures, antibiogram.
Hospital Internship: Hipolito Unanue Hospital (6 months).

PUBLICATIONS

  • Zhu P, Fajardo O, Shum J, Zhang Scharer YP, Friedrich RW: “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device”, Nature Protocols, 2012, Jun 28;7(7):1410-1425.
  • Zhu P, Narita Y, Bundschuh ST, Fajardo O, Scharer YP, Chattopadhyaya B, Bouldoires EA, Stepien AE, Deisseroth K, Arber S, Sprengel R, Rijli FM, Friedrich RW: “Optogenetic Dissection of Neuronal Circuits in Zebrafish using Viral Gene Transfer and the Tet System”, Frontiers in Neural Circuits, 2009 Dec 11; 3:21.
  • Fajardo O, Meseguer V, Belmonte C, Viana F: “TRPA1 Channels, target of 1,4-dihydropyridines”, Channels (Austin), 2008 Nov-Dec;2(6):429-38.
  • Fajardo O, Meseguer V, Belmonte C, Viana F: “TRPA1 Channels Mediate Cold Temperature Sensing in Mammalian Vagal Sensory Neurons: Pharmacological and Genetic Evidence” The Journal of Neuroscience, 2008 28(31):7863-7875.
  • Nunez E, Benito C, Pazos R, Barbachano A, Fajardo O, Gonzalez S, Tolon R, Romero J: “Cannabinoid CB2 Receptors Are Expressed by Perivascular Microglial Cells in the Human Brain: An Immunohistochemical Study” Synapse, 2004 53:208–213.

In preparation:

  • Fajardo O, Zhu P, Friedrich RW: “High-level neural control of a defined motor pattern in zebrafish: an optogenetic analysis”.
  • Lopez Gonzalez MJ, Fajardo O, Meseguer V, Valero M, Pertusa M, Belmonte C. & Viana F. “Phenytoin, nifedipine and carbamazepine induce gingival enlargement trough TRPA1 activation”.
  • Meseguer V, Alpizar YA ,  Fajardo O, Tajada S, Denlinger B, Talavera A,  Luis E.,  Manenschjin J, Perez-Garcia MT,   Lopez JR,  Belmonte C, Talavera K, Viana F: ‘TRPA1 Channels are neuronal sensors for bacterial endotoxins’. (submitted)

 

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